Difference between revisions of "Part:BBa S03724"

Latest revision as of 06:25, 27 October 2007

B0015:S03708
A weak (as compared to J23100) constitutive promoter J23116 produces a steady stream of the tet repressor protein. When cloned into other constructs containing pTet, this tetR will constantly repress transcription of the pTet-regulated gene. This intermediate also contains a B0015 terminator upstream of the tetR producing region, so that pTet-spacer-cro/N/Q/YFP constructs can be easily cloned upstream of the terminator, resulting in the final testing construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 144
Illegal NheI site found at 167
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_S03724 short</partinfo>
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-A weak (as compared to J231) constitutive promoter J23116 produces a steady stream of the tet repressor protein. When cloned into other constructs containing pTet, this tetR will constantly repress transcription of the pTet-regulated gene. This intermediate also contains a B0015 terminator upstream of the tetR producing region, so that pTet-spacer-cro/N/Q/YFP constructs can be easily cloned upstream of the terminator, resulting in the final testing construct.
+A weak (as compared to J23100) constitutive promoter J23116 produces a steady stream of the tet repressor protein. When cloned into other constructs containing pTet, this tetR will constantly repress transcription of the pTet-regulated gene. This intermediate also contains a B0015 terminator upstream of the tetR producing region, so that pTet-spacer-cro/N/Q/YFP constructs can be easily cloned upstream of the terminator, resulting in the final testing construct.
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