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Latest revision as of 04:39, 27 October 2007

Constitutive Q

The pTet promoter, in the absence of tet repressor, produces Q transcripts. As the transcript now contains a terminator, the cell will translate the transcript into Q protein. Because the construct does not contain any tetR producing elements, this construct will result in the maximum concentration of Q protein production (per copy of the gene) in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]