Difference between revisions of "Part:BBa I759030"
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The pTet promoter, in the absence of tet repressor, produces N transcripts. As the transcript now contains a terminator, the cell will translate the transcript into N protein. Because the construct does not contain any tetR producing elements, this construct will result in the maximum concentration of N protein production (per copy of the gene) in the cell. | The pTet promoter, in the absence of tet repressor, produces N transcripts. As the transcript now contains a terminator, the cell will translate the transcript into N protein. Because the construct does not contain any tetR producing elements, this construct will result in the maximum concentration of N protein production (per copy of the gene) in the cell. | ||
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Revision as of 04:37, 27 October 2007
Constitutive N
The pTet promoter, in the absence of tet repressor, produces N transcripts. As the transcript now contains a terminator, the cell will translate the transcript into N protein. Because the construct does not contain any tetR producing elements, this construct will result in the maximum concentration of N protein production (per copy of the gene) in the cell.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]