Difference between revisions of "Part:BBa I759028"
 
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<partinfo>BBa_I759028 short</partinfo>
 
<partinfo>BBa_I759028 short</partinfo>
  
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The part has the cis-repressor cis4 cloned into the spacer region of I759003.
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This part has the Q construct from I759003 with the cis repressor cis4 cloned in to replace the spacer. The weak constitutive promoter J23116 will produce a steady stream of tetR, which (in the absence of aTc) repress transcription of the pTet-cis4-Q transcript. The riboregulator design further represses levels of Q protein in the cell. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.  
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Latest revision as of 04:33, 27 October 2007

cis4-repressed, tet-regulated Q

This part has the Q construct from I759003 with the cis repressor cis4 cloned in to replace the spacer. The weak constitutive promoter J23116 will produce a steady stream of tetR, which (in the absence of aTc) repress transcription of the pTet-cis4-Q transcript. The riboregulator design further represses levels of Q protein in the cell. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 890
    Illegal NheI site found at 913
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]