Difference between revisions of "Part:BBa I759002"
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A pTet promoter (can be repressed by tet Repressor, and transcriptional activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it. The finished construct can produce an aTc and cis-dependent level of cro protein. | A pTet promoter (can be repressed by tet Repressor, and transcriptional activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it. The finished construct can produce an aTc and cis-dependent level of cro protein. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 04:18, 27 October 2007
pTet-spacer-cro
pTet-spacer-cro
A pTet promoter (can be repressed by tet Repressor, and transcriptional activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in. This construct is an intermediate, as the terminator sequence for the transcript has not yet been cloned it. The finished construct can produce an aTc and cis-dependent level of cro protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]