Difference between revisions of "Part:BBa I759001:Design"
(→Design Notes) |
(→Source) |
||
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | pTet is a standard iGEM promoter, and the N DNA sequence came from the genome of commercial lambda zap virus. The two parts were connected with an intervening spacer through the process of overlap extension PCRs, using specially designed spacer-primers. | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 04:15, 27 October 2007
pTet-spacer-N
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pTet promoter regulates N transcript production, and transcription can be repressed by tet repressor, or re-activated by the addition of aTc. This construct is an intermediate, as it is missing the terminator for the transcript. The spacer acts as a placeholder for cis sequences which will be cloned in in the future.
Source
pTet is a standard iGEM promoter, and the N DNA sequence came from the genome of commercial lambda zap virus. The two parts were connected with an intervening spacer through the process of overlap extension PCRs, using specially designed spacer-primers.