Difference between revisions of "Part:BBa I759001:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The pTet promoter regulates N transcript production, and transcription can be repressed by tet repressor, or re-activated by the addition of aTc. This construct is an intermediate, as it is missing the terminator for the transcript. The spacer acts as a placeholder for cis sequences which will be cloned in in the future.
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===Source===
 
===Source===

Revision as of 04:13, 27 October 2007


pTet-spacer-N


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pTet promoter regulates N transcript production, and transcription can be repressed by tet repressor, or re-activated by the addition of aTc. This construct is an intermediate, as it is missing the terminator for the transcript. The spacer acts as a placeholder for cis sequences which will be cloned in in the future.

Source

Caltech iGEM

References