Difference between revisions of "Part:BBa K2810010"
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A promoter sequence that is activated by Ribitol, an ribose-derived compound. This part is GoldenGate compatible.
 
A promoter sequence that is activated by Ribitol, an ribose-derived compound. This part is GoldenGate compatible.
  
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The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that makes the sequence conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.  
+
The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:40, 3 September 2018


Regulatory region derived from the ribitol-responsive operon

A promoter sequence that is activated by Ribitol, an ribose-derived compound. This part is GoldenGate compatible.

The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 57
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]