Difference between revisions of "Part:BBa I751201"

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pcI-RBS-luxI on pSB4A1.
 
pcI-RBS-luxI on pSB4A1.
  
We needed data which ensures that the amount of AHL is well enough to 'turn on' our hybrid promoter, even if LuxI is on a low copy plasmid.
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We used this part to confirm whether a low copy number of plasmid used in our project can produce enought AHL to activate the lux lac hybrid promoter.
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:58, 27 October 2007

pcI-luxI on pSB4

pcI-RBS-luxI on pSB4A1.

We used this part to confirm whether a low copy number of plasmid used in our project can produce enought AHL to activate the lux lac hybrid promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 740
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]