Difference between revisions of "Part:BBa K2810009"
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<partinfo>BBa_K2810009 short</partinfo> | <partinfo>BBa_K2810009 short</partinfo> | ||
| − | The sequence of mCherry, derived from part BBa_K1668011, but codon optimised for <i> Nicotiana benthamiana </i>. It has extra 5' and 3' sites that make it GoldenGate Compatible. | + | The sequence of mCherry, derived from part BBa_K1668011, but codon optimised for <i> Nicotiana benthamiana </i>. It has extra 5' and 3' sites that make it GoldenGate Compatible. This is because there were no RFPs or derivatives in the iGEM parts registry that were GoldenGate compatible. This part expands the phytobrick registry and can be a powerful tool for plant cell imaging. RFP and its derivatives fluoresce in a different wavelength of light relative to GFP, which fluoresces in a similar wavelength to chlorophyll, and thereby is unfavourable to use a reporter gene in plants. mCherry also functions as a monomer, rather than a dimer like GFP, and consequently often has more consistent and higher levels of expression. This is an improved part. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 11:10, 31 August 2018
Status: 500
Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
The sequence of mCherry, derived from part BBa_K1668011, but codon optimised for Nicotiana benthamiana . It has extra 5' and 3' sites that make it GoldenGate Compatible. This is because there were no RFPs or derivatives in the iGEM parts registry that were GoldenGate compatible. This part expands the phytobrick registry and can be a powerful tool for plant cell imaging. RFP and its derivatives fluoresce in a different wavelength of light relative to GFP, which fluoresces in a similar wavelength to chlorophyll, and thereby is unfavourable to use a reporter gene in plants. mCherry also functions as a monomer, rather than a dimer like GFP, and consequently often has more consistent and higher levels of expression. This is an improved part.
Sequence and Features Status: 500 Content-type: text/html
Software error:
Global symbol "$range" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 250. Unmatched right curly bracket at /websites/parts.igem.org/cgi/lib/Range.pm line 251, at end of line syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 251, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 261, near "= @_" syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 282, near "}" Can't use global @_ in "my" at /websites/parts.igem.org/cgi/lib/Range.pm line 286, near "= @_" Global symbol "$course" requires explicit package name at /websites/parts.igem.org/cgi/lib/Range.pm line 288. syntax error at /websites/parts.igem.org/cgi/lib/Range.pm line 314, near "}" Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 16. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 16. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.