Difference between revisions of "Part:BBa I751021"

Revision as of 03:29, 27 October 2007

plac-lux-cI-mCherry In our project this part is used on the A side. Expression is detected with the fluorescence protein mCherry and repressed by lambda cI.

This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.

This part is for our second plan, in case BBa_I751011 doesn't work.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5246
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2762
Illegal SpeI site found at 5247
Illegal PstI site found at 5261
Illegal NotI site found at 2768
Illegal NotI site found at 5254
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2762
Illegal BglII site found at 3016
Illegal BglII site found at 3698
Illegal BamHI site found at 2798
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5247
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 2762
Illegal XbaI site found at 2777
Illegal SpeI site found at 5247
Illegal PstI site found at 5261
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2003
Illegal SapI site found at 920

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I751021 short</partinfo>
-This is made for A-side plasmid in our project.
+In our project this part is used on the A side. Expression is detected with the fluorescence protein mCherry and repressed by lambda cI.
-Regulated by lac repressor, this part makes LuxI, cI repressor, and mCherry (for detecting).
+This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.
-If needed, you can move this part into B-side plasmid (by DraIII or AatII), regulating the rate of cI/lac repressor.
 This part is for our second plan, in case BBa_I751011 doesn't work.