Difference between revisions of "Part:BBa I714051:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Simply ligate the three parts sequentially to test the expression after tandem oriT is truncated. | |
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===Source=== | ===Source=== |
Latest revision as of 03:19, 27 October 2007
[I714030][J23066][I714030]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 278
Illegal NheI site found at 324
Illegal NheI site found at 985
Illegal NheI site found at 1031 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 484
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 361
Design Notes
Simply ligate the three parts sequentially to test the expression after tandem oriT is truncated.
Source
[I714030] + [J23066] + [I714030]