Difference between revisions of "Part:BBa K2810000"
 
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<partinfo>BBa_K2810000 short</partinfo>
 
<partinfo>BBa_K2810000 short</partinfo>
  
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This part contains the sequence of the WRKYintron, which is commonly used as to separate the sense and anti-sense parts of an RNAi contruct for [https://currentprotocols.onlinelibrary.wiley.com/doi/abs/10.1002/0471142727.mb2606s72%4010.1002/%28ISSN%291934-3647.GeneSilencing gene silencing]. This has been used many times by plant biologists and is part of the [https://www.uniprot.org/taxonomy/188084 pJawohl8-RNAi binary plasmid].
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This part contains the sequence of the WRKYintron, which is commonly used as to separate the sense and anti-sense parts of an RNAi contruct for gene silencing. This has been used many times by plant biologists and is part of the pJawohl8-RNAi binary plasmid.
  
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The WRKYintron is cloned into the the golden gate version of pSB1C3 ([https://parts.igem.org/Part:BBa_P10500 P10500]) using BsmBI and conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick standard]. It is flanked by BsaI sites together 5' CCAT and 3' AATG cloning sequences for subsequent cloning into a level 1 plasmid.
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The WRKYintron is cloned into the the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the Phytobrick standard. It is flanked by BsaI sites together 5' CCAT and 3' AATG cloning sequences for subsequent cloning into a level 1 plasmid.
  
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This has been used to generate the composite RNAi constructs detailed in the 2018 Cardiff_Wales Parts page  
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This has been used to generate the RNAi constructs detailed in the 2018 Cardiff_Wales Parts page  
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ADD WHICH COMPOSITE CLONES
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:59, 24 August 2018


WRKY intron for plant-targeted RNAi constructs

This part contains the sequence of the WRKYintron, which is commonly used as to separate the sense and anti-sense parts of an RNAi contruct for gene silencing. This has been used many times by plant biologists and is part of the pJawohl8-RNAi binary plasmid.

The WRKYintron is cloned into the the golden gate version of pSB1C3 (P10500) using BsmBI and conforms to the Phytobrick standard. It is flanked by BsaI sites together 5' CCAT and 3' AATG cloning sequences for subsequent cloning into a level 1 plasmid.

This has been used to generate the RNAi constructs detailed in the 2018 Cardiff_Wales Parts page

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]