Difference between revisions of "Part:BBa J100397"
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J100397 (pClone mScarlet) is a modification of pClone Red [https://parts.igem.org/Part:BBa_J119137 J119137]. We removed RFP and inserted mScarlet using BamHI on the 5' end of mScarlet, and PstI on the 3' end. This device is [https://www.lifescied.org/doi/10.1187/cbe.13-09-0189 ideal for testing new promoters] and has been used for course base undergraduate research experiences (CUREs).  
 
J100397 (pClone mScarlet) is a modification of pClone Red [https://parts.igem.org/Part:BBa_J119137 J119137]. We removed RFP and inserted mScarlet using BamHI on the 5' end of mScarlet, and PstI on the 3' end. This device is [https://www.lifescied.org/doi/10.1187/cbe.13-09-0189 ideal for testing new promoters] and has been used for course base undergraduate research experiences (CUREs).  
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Revision as of 14:31, 5 August 2018


pClone mScarlet

J100397 (pClone mScarlet) is a modification of pClone Red J119137. We removed RFP and inserted mScarlet using BamHI on the 5' end of mScarlet, and PstI on the 3' end. This device is ideal for testing new promoters and has been used for course base undergraduate research experiences (CUREs).

J100397.png

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 942
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 942
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 942
    Illegal BamHI site found at 888
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 942
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 816
    Illegal BsaI.rc site found at 754