Difference between revisions of "Part:BBa K2688003:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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Simplest translational unit design, with some spacers for increased expression reliability.
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Simplest translational unit design. In our actual design, we added some some spacers for increased expression reliability : 8 bases from +1 of transcription to the RBS beginning, 10 between the CDS and terminator, and 33 after the terminator (before the suffix).
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+
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===Source===
 
===Source===

Latest revision as of 16:22, 1 August 2018


cpg2_tu


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 132
    Illegal NgoMIV site found at 820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 919


Design Notes

Simplest translational unit design. In our actual design, we added some some spacers for increased expression reliability : 8 bases from +1 of transcription to the RBS beginning, 10 between the CDS and terminator, and 33 after the terminator (before the suffix).

Source

All parts are existing iGEM registry biobrick part

References