Difference between revisions of "Part:BBa J119409"

 
 
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<partinfo>BBa_J119409 short</partinfo>
 
<partinfo>BBa_J119409 short</partinfo>
  
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This device supports a project that will use the SmaI gene as a fitness gene during in vitro gene expression experiments. It uses a T7 RNA polymerase promoter to express the SmaI gene. SmaI protein is expected to cut the template that made it in two places, which will free it from scaffolding used during the in vitro gene expression experiments.
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This device supports a project that will use the SmaI gene as a fitness gene during in vitro gene expression experiments. It uses a T7 RNA polymerase promoter to express the SmaI gene. SmaI protein is expected to cut the template that made it in two places, which will free it from scaffolding used during the in vitro gene expression experiments. Deleted G in position 300 of the construct.
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Source
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:38, 13 July 2018


SmaI Restriction Endonuclease Expression Device

This device supports a project that will use the SmaI gene as a fitness gene during in vitro gene expression experiments. It uses a T7 RNA polymerase promoter to express the SmaI gene. SmaI protein is expected to cut the template that made it in two places, which will free it from scaffolding used during the in vitro gene expression experiments. Deleted G in position 300 of the construct.

Source


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 299
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 641