Difference between revisions of "Part:BBa J100435"

 
 
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<partinfo>BBa_J100435 short</partinfo>
 
<partinfo>BBa_J100435 short</partinfo>
  
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modified actClone [https://parts.igem.org/Part:BBa_J100204 (J100204)] with basic strong RBSs replacing the Cdog RBSs, no transcriptional terminator, and BsaI sites that cut out the GFP along with the plasmid's original reverse promoter and reverse RBS.  
+
modified actClone [https://parts.igem.org/Part:BBa_J100204 (J100204)] with basic strong RBSs replacing the Cdog RBSs, the transcriptional terminator removed, and BsaI sites that cut out the GFP along with the plasmid's original reverse promoter and reverse RBS.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:12, 12 July 2018


actClone 2 Red

modified actClone (J100204) with basic strong RBSs replacing the Cdog RBSs, the transcriptional terminator removed, and BsaI sites that cut out the GFP along with the plasmid's original reverse promoter and reverse RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1556
    Illegal AgeI site found at 1429
    Illegal AgeI site found at 1541
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 806
    Illegal BsaI.rc site found at 12
    Illegal SapI site found at 719