Difference between revisions of "Part:BBa K2680251"

 
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<partinfo>BBa_K2680251 short</partinfo>
 
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Coding Sequence for red fluorescent protein mScarlet-I in Team William_and_Mary iGEM 2018's 3G format. This part is also compatible with CIDAR MoClo assembly. mScarlet-I is  the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 . mScarlet-I is distinguished from the original mScarlet protein by a single amino acid substitution (T74I). mScarlet-I has a peak excitation at 569nm and peak emission at 594nm, and possesses both a high quantum yield (0.54) and fluorescence lifetime (3.1ns), and a rapid maturation time of ~36 minutes.  
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Coding Sequence for red fluorescent protein mScarlet-I in Team William_and_Mary iGEM 2018's 3G format. This part is also compatible with CIDAR MoClo assembly. mScarlet-I is  the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 . mScarlet-I is distinguished from the original mScarlet protein by a single amino acid substitution (T74I). mScarlet-I has a peak excitation at 569nm and peak emission at 594nm, and possesses both a high quantum yield (0.54) and fluorescence lifetime (3.1ns), and a rapid maturation time of ~36 minutes [1].
  
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== Reference ==
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[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M. Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074
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Revision as of 20:19, 7 July 2018


3G mScarlet-I

Coding Sequence for red fluorescent protein mScarlet-I in Team William_and_Mary iGEM 2018's 3G format. This part is also compatible with CIDAR MoClo assembly. mScarlet-I is the rapidly-maturing version of mScarlet, a monomeric red fluorescent protein designed by Bindels et al. in 2016 . mScarlet-I is distinguished from the original mScarlet protein by a single amino acid substitution (T74I). mScarlet-I has a peak excitation at 569nm and peak emission at 594nm, and possesses both a high quantum yield (0.54) and fluorescence lifetime (3.1ns), and a rapid maturation time of ~36 minutes [1].

Reference


[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M. Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 574
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 49
    Illegal BsaI.rc site found at 778