Difference between revisions of "Part:BBa K2680500"
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<partinfo>BBa_K2680500 short</partinfo> | <partinfo>BBa_K2680500 short</partinfo> | ||
| − | Orthoganol flanker sequence on the prefix side of all William_and_Mary iGEM 2018 | + | Orthoganol flanker sequence on the prefix side of all 3G Assembly parts from William_and_Mary iGEM 2018. Along with <partinfo>BBa_K2680501</partinfo>, this allows easy PCR of inserts for confirmation of colonies or for the generation of additional DNA for 3G assembly (without miniprep). Further, these WM_Pad sequences allow for high-quality PCR of iGEM backbones for use in Gibson Assembly. Both WM_Pad sequences are free of BsmBI and BsaI sites and so are compatible with most Golden Gate Assembly platforms. Additionally, the WM_Pad sequences are orthogonal to the <i>E. coli</i> genome and to the UNS sequences used in 3G assembly. |
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Latest revision as of 18:57, 6 July 2018
WM_Pad1
Orthoganol flanker sequence on the prefix side of all 3G Assembly parts from William_and_Mary iGEM 2018. Along with BBa_K2680501, this allows easy PCR of inserts for confirmation of colonies or for the generation of additional DNA for 3G assembly (without miniprep). Further, these WM_Pad sequences allow for high-quality PCR of iGEM backbones for use in Gibson Assembly. Both WM_Pad sequences are free of BsmBI and BsaI sites and so are compatible with most Golden Gate Assembly platforms. Additionally, the WM_Pad sequences are orthogonal to the E. coli genome and to the UNS sequences used in 3G assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]