Difference between revisions of "Part:BBa K2680500:Design"

 
 
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===Design Notes===
 
===Design Notes===
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This part was designed so that it would be orthogonal to the<i>E. coli</i> genome, the other WM_Pad site and the UNS sequences used in 3G assembly. GC content was set to be between 45-55%, and the sequences for forbidden or common restriction sites were forbidden. Tracks of identical base pairs longer than 3 were disallowed, and sequences needed to end in a G or C base.  
+
This part was designed so that it would be orthogonal to the <i>E. coli</i> genome, the other WM_Pad site and the UNS sequences used in 3G assembly. GC content was set to be between 45-55%, and the sequences for forbidden or common restriction sites were forbidden. Tracks of identical base pairs longer than 3 were disallowed, and sequences needed to end in a G or C base.  
  
  

Latest revision as of 18:49, 6 July 2018


WM_Pad1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was designed so that it would be orthogonal to the E. coli genome, the other WM_Pad site and the UNS sequences used in 3G assembly. GC content was set to be between 45-55%, and the sequences for forbidden or common restriction sites were forbidden. Tracks of identical base pairs longer than 3 were disallowed, and sequences needed to end in a G or C base.


Source

This part's sequence was designed BY EMJ using https://www.syntegron.org/R20/R20/R20.html (2013), and was added to iGEM vectors using primers synthesized from IDT.

References