Difference between revisions of "Part:BBa J100413"
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| − | Gal4 binding site inserted upstream of a T7 promoter consensus sequence [https://parts.igem.org/Part:BBa_J100402 (J100402)] that will produce RFP when inserted into pClone [https://parts.igem.org/Part:BBa_J119137 (J119137)]. When present the Gal4 protein will bind to | + | Gal4 binding site inserted upstream of a T7 promoter consensus sequence [https://parts.igem.org/Part:BBa_J100402 (J100402)] that will produce RFP when inserted into pClone [https://parts.igem.org/Part:BBa_J119137 (J119137)]. When present the Gal4 protein will bind to the site. |
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Latest revision as of 19:57, 3 July 2018
T7 Promoter with Upstream Gal4 Binding Site in pClone Red
Gal4 binding site inserted upstream of a T7 promoter consensus sequence (J100402) that will produce RFP when inserted into pClone (J119137). When present the Gal4 protein will bind to the site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1454
Illegal AgeI site found at 1566 - 1000COMPATIBLE WITH RFC[1000]