Difference between revisions of "Part:BBa J100432"

 
 
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<partinfo>BBa_J100432 short</partinfo>
 
<partinfo>BBa_J100432 short</partinfo>
  
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T7 consensus sequence [https://parts.igem.org/Part:BBa_R0085 (R0085)] inserted into pClone Red [https://parts.igem.org/Part:BBa_J100397 (J119137)], encoding for mScarlet. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.
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T7 consensus sequence [https://parts.igem.org/Part:BBa_R0085 (R0085)] inserted into pClone mScarlet [https://parts.igem.org/Part:BBa_J100397 (J119137)], encoding for mScarlet. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.
  
 
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Latest revision as of 20:13, 29 June 2018


T7 Consensus Promoter in pClone mScarlet

T7 consensus sequence (R0085) inserted into pClone mScarlet (J119137), encoding for mScarlet. Used as a control to test against experimental promoters that had Gal4 binding sites upstream, downstream, and within this promoter sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 895
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 895
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 895
    Illegal BamHI site found at 841
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 895
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 895
  • 1000
    COMPATIBLE WITH RFC[1000]