Difference between revisions of "Part:BBa J100377"

Latest revision as of 13:42, 12 June 2018

rClone Red with Riboswitch C Mutant #3

This is an rClone Red plasmid (J119384) that replaces the GFP cassette with the riboswitch C (RS-C) mutant #3. This mutant was produced by evolving RS-C, which turns "on" in the presence of theophylline, with phage assisted continuous evolution (PACE) in an attempt to create an RS-C mutant that responds to xanthine instead of theophylline. The RS-C mutant #3 sequence is one of ten RS-C mutants tested for xanthine specificity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 662
Illegal AgeI site found at 774
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_J100377 short</partinfo>
-This is an rClone Red plasmid (J199384) that replaces the GFP cassette with the riboswitch C (RS-C) mutant #3. This mutant was produced by evolving RS-C, which turns "on" in the presence of theophylline, with phage assisted continuous evolution (PACE) in an attempt to create an RS-C mutant that responds to xanthine instead of theophylline. The RS-C mutant #3 sequence is one of ten RS-C mutants tested for xanthine specificity.
+This is an rClone Red plasmid (J119384) that replaces the GFP cassette with the riboswitch C (RS-C) mutant #3. This mutant was produced by evolving RS-C, which turns "on" in the presence of theophylline, with phage assisted continuous evolution (PACE) in an attempt to create an RS-C mutant that responds to xanthine instead of theophylline. The RS-C mutant #3 sequence is one of ten RS-C mutants tested for xanthine specificity.
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