Difference between revisions of "Part:BBa M50433"

Latest revision as of 06:15, 12 June 2018

AtAAE3 coding sequence (yeast optimized) for oxalate catabolism

This part codes an oxalyl-CoA synthetase from Arabidopsis thaliana, with a HIS Tag at the end. This enzyme catabolizes oxalate to oxalyl-CoA, which is the first step in the pathway for total oxalate catabolism. This part contains the coding sequence for AtAAE3, with codons optimized for usage in Saccharomyces cerevisiae. Combine this part with a promoter, Kozak consensus sequence, and a polyA tail. The coding sequence already contains a 6x HIS tag. The sequence for the AtAAE3 gene was derived from the European Nucleotide Archive via UniProtKB (UniProtKB Entry: Q9SMT7). The actual sequence was submitted by Salanoubat et al.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 286
Illegal SpeI site found at 526
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 286
Illegal SpeI site found at 526
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 286
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 286
Illegal SpeI site found at 526
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 286
Illegal SpeI site found at 526
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_M50433 short</partinfo>
-This part codes an oxalyl-CoA synthetase from Arabidopsis thaliana, with a HIS Tag at the end. This enzyme catabolizes oxalate to oxalyl-CoA, which is the first step in the pathway for total oxalate catabolism. This part contains the coding sequence for AtAAE3, with codons optimized for usage in Saccharomyces cerevisiae. Combine this part with a promoter, Kozak consensus sequence, and a polyA tail. The coding sequence already contains a 6x HIS tag.
+This part codes an oxalyl-CoA synthetase from Arabidopsis thaliana, with a HIS Tag at the end. This enzyme catabolizes oxalate to oxalyl-CoA, which is the first step in the pathway for total oxalate catabolism. This part contains the coding sequence for AtAAE3, with codons optimized for usage in Saccharomyces cerevisiae. Combine this part with a promoter, Kozak consensus sequence, and a polyA tail. The coding sequence already contains a 6x HIS tag. The sequence for the AtAAE3 gene was derived from the European Nucleotide Archive via UniProtKB (UniProtKB Entry: Q9SMT7). The actual sequence was submitted by Salanoubat et al.
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