Difference between revisions of "Part:BBa M50420:Design"
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===Design Notes===
 
===Design Notes===
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We codon optimized the amino acid sequence for E. coli. We did not make any other modifications from the source sequences.  
+
We codon optimized the Cry11Aa amino acid sequence for E. coli. All of our parts were sourced from existing iGEM parts which are listed below. We did not make any other modifications from the source sequences.  
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+
  
  
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The source is previously established parts and the protein from uniprot cry11Aa.
 
The source is previously established parts and the protein from uniprot cry11Aa.
  
−
Promoter:
+
Promoter: BBa_K914003
  
−
RBS:
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RBS: BBa_B0034
  
−
Leader Sequence:
+
Leader Sequence: BBa_J32015
  
−
Gene:  
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Gene: BBa_K332011 codon optimized for E. coli
  
−
6x-His Tag:  
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6x-His Tag: BBa_K1223006
  
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Terminator:
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Terminator: BBa_B0012
  
  
 
===References===
 
===References===

Revision as of 03:23, 12 June 2018


Mosquito Larvae Insecticide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 188
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized the Cry11Aa amino acid sequence for E. coli. All of our parts were sourced from existing iGEM parts which are listed below. We did not make any other modifications from the source sequences.


Source

The source is previously established parts and the protein from uniprot cry11Aa.

Promoter: BBa_K914003

RBS: BBa_B0034

Leader Sequence: BBa_J32015

Gene: BBa_K332011 codon optimized for E. coli

6x-His Tag: BBa_K1223006

Terminator: BBa_B0012


References