Difference between revisions of "Part:BBa I13522"
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Image:I13522 - UV 254nm.JPG|BBa_I13522 visualized under 254nm wavelength UV lightbox
 
Image:I13522 - UV 254nm.JPG|BBa_I13522 visualized under 254nm wavelength UV lightbox
 
</gallery>
 
</gallery>
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==Characterization of [[Chassis/Cell-Free_Systems|Cell-Free Chassis]]==
 +
This part was used to characterize the cell-free chassis. Details of characterization can be found on the [[Chassis/Cell-Free Systems/Commercial E.coli S30|cell-free characterization]] page. Other interesting isothermal properties of this construct are available at the [http://parts.mit.edu/igem07/index.php/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.
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{|border=1 width="90%" align="center"
 +
|-
 +
!width="20%" style="background:#CCCCFF"|Parameter
 +
!width="80%"|Value and Description
 +
|-align="center"
 +
|style="background:#EEEEFF"|Optimal Temperature
 +
|Expression increases with temperature from 4&deg;C to 30&deg;C
 +
|-align="center"
 +
|style="background:#EEEEFF"|Optimal DNA Concentration
 +
|Works optimally with 2&micro;g of DNA in 60 &micro;l of cell-extract
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|-align="center"
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|style="background:#EEEEFF"|Peak Expression Time
 +
|Peak rate of output occurs after approximately 85 minutes
 +
|-align="center"
 +
|style="background:#EEEEFF"|Expression Lifespan
 +
|Expression levels off after 2 hours
 +
|-align="center"
 +
|style="background:#EEEEFF"|Expression Capacity
 +
|Can synthesize over 200pmols of GFPmut3b in 6 hours
 +
|}

Revision as of 00:10, 27 October 2007

pTet GFP

Untagged GFP behind a constitutive promoter.

Usage and Biology

GFP only control

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Pictures

  • BBa_I13522 visualized under non-UV lightbox

  • BBa_I13522 visualized under 254nm wavelength UV lightbox

Characterization of Cell-Free Chassis

This part was used to characterize the cell-free chassis. Details of characterization can be found on the cell-free characterization page. Other interesting isothermal properties of this construct are available at the [http://parts.mit.edu/igem07/index.php/Imperial/Cell_by_Date/Testing Cell By Date project page] of Imperial 2007 wiki.

Parameter Value and Description
Optimal Temperature Expression increases with temperature from 4°C to 30°C
Optimal DNA Concentration Works optimally with 2µg of DNA in 60 µl of cell-extract
Peak Expression Time Peak rate of output occurs after approximately 85 minutes
Expression Lifespan Expression levels off after 2 hours
Expression Capacity Can synthesize over 200pmols of GFPmut3b in 6 hours