Difference between revisions of "Part:BBa M50436:Design"
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===Design Notes===
 
===Design Notes===
−
We acquired the base pair sequence for this protein by running the uniprot amino acid sequence for ahpC through the Integrated DNA Technologies (IDT) Codon Optimization software, optimized for ''E. coli''. We then added a FLAG sequence at the C-terminus so that our protein could be easily visualized using a Western Blot.
+
We acquired the base pair sequence for this protein by running the UniProt amino acid sequence for ahpC through the Integrated DNA Technologies (IDT) Codon Optimization software, optimized for ''E. coli''. We then added a FLAG sequence at the C-terminus so that our protein could be easily visualized using a Western Blot.
  
  

Revision as of 23:45, 11 June 2018


ahpC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 58
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 58
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We acquired the base pair sequence for this protein by running the UniProt amino acid sequence for ahpC through the Integrated DNA Technologies (IDT) Codon Optimization software, optimized for E. coli. We then added a FLAG sequence at the C-terminus so that our protein could be easily visualized using a Western Blot.


Source

Uniprot Amino Acid Sequence: https://www.uniprot.org/uniprot/P0AE08

FLAG sequence: BBa_T2004


References

https://www.uniprot.org/uniprot/P0AE08

https://parts.igem.org/Part:BBa_T2004

Ma, L. & Payne, S. “AhpC is Required for Optimal Production of Enterobactin by Escherichia coli”. Journal of Bacteriology, 2012. http://jb.asm.org/content/194/24/6748.long