Difference between revisions of "Part:BBa M50436:Experience"
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| − | We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. | + | We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into E. coli, then plated the E. coli on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized. |
Revision as of 23:40, 11 June 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M50436
This protein can be used to speed up enterobactin synthesis slightly.
User Reviews
UNIQ2afdfa1a30fd6e24-partinfo-00000000-QINU UNIQ2afdfa1a30fd6e24-partinfo-00000001-QINU
We used this sequence as a part of a 2,3-DHB Biosynthesis construct (Part:BBa_M50437). We ordered the plasmid from DNA2.0 and transformed it into E. coli, then plated the E. coli on LB + kanamycin. We saw colonies successfully grow up. We also ran a Western Blot for this protein and a saw a band of the expected size, indicating this protein was successfully synthesized.