Difference between revisions of "Part:BBa M50440:Experience"
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| + | ===Stanford Location=== | ||
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| + | Plasmid name: pHlash<BR> | ||
| + | DNA2.0 Gene #: custom version of <BR> | ||
| + | Organism: S. cerevisiae <BR> | ||
| + | Device type: Sensor<BR> | ||
| + | Glycerol stock barcode: 133021752<BR> | ||
| + | Box label: BioE44 S18 | ||
Revision as of 17:45, 11 June 2018
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_M50440
This construct can successfully be used to measure intracellular pH given by a BRET ratio in S. cerevisiae. Due to the well-characterized pH-sensitivity of Rluc8 (luciferase), we are confident that BRET readout from cpVenus (fluorophore) is indicative of intracellular pH because it was excited through Rluc8 activity.
User Reviews
UNIQ91f7b090d9580b65-partinfo-00000000-QINU UNIQ91f7b090d9580b65-partinfo-00000001-QINU
Stanford Location
Plasmid name: pHlash
DNA2.0 Gene #: custom version of
Organism: S. cerevisiae
Device type: Sensor
Glycerol stock barcode: 133021752
Box label: BioE44 S18