Difference between revisions of "Part:BBa M50440:Design"
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+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_M50440 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | ===Design Notes=== | ||
+ | |||
+ | |||
+ | |||
+ | ===Source=== | ||
+ | |||
+ | GAL1 promoter sequence: BBa_J63006 | ||
+ | |||
+ | pHlash sequence: pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH, PLOS (BBa_M50440) | ||
+ | |||
+ | Terminator sequence: BBa_K1462070 | ||
+ | |||
+ | ===References=== | ||
+ | |||
+ | |||
+ | https://parts.igem.org/Part:BBa_J63006 | ||
+ | <BR> | ||
+ | <BR> | ||
+ | https://parts.igem.org/Part:BBa_K1462070 | ||
+ | <BR> | ||
+ | <BR> | ||
+ | Zhang, Yunfei et al. “pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH.” Ed. Valentin Ceña. PLoS ONE 7.8 (2012): e43072. PMC. Web. 9 May 2018. | ||
+ | <BR> | ||
+ | <BR> |
Revision as of 17:29, 11 June 2018
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
Illegal AgeI site found at 759
Illegal AgeI site found at 1648 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1500
Illegal BsaI.rc site found at 1194
Illegal SapI.rc site found at 1513
Design Notes
Source
GAL1 promoter sequence: BBa_J63006
pHlash sequence: pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH, PLOS (BBa_M50440)
Terminator sequence: BBa_K1462070
References
https://parts.igem.org/Part:BBa_J63006
https://parts.igem.org/Part:BBa_K1462070
Zhang, Yunfei et al. “pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH.” Ed. Valentin Ceña. PLoS ONE 7.8 (2012): e43072. PMC. Web. 9 May 2018.