Difference between revisions of "Part:BBa I741001:Experience"
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The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below). | The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below). | ||
| − | + | [[Image:Image:Swarming cells induced with AHL.JPG]] | |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 23:24, 26 October 2007
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_I741001
The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below). File:Image:Swarming cells induced with AHL.JPG
User Reviews
UNIQ616896482bf722de-partinfo-00000000-QINU UNIQ616896482bf722de-partinfo-00000001-QINU