Difference between revisions of "Part:BBa I741001:Experience"
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===Applications of BBa_I741001=== | ===Applications of BBa_I741001=== | ||
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| + | The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below). | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 23:23, 26 October 2007
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Applications of BBa_I741001
The HSL Receiver construct restores motility in motB knockout strain RP3087 on induction with HSL. The plasmid is sensitive to HSL concentrations as low as 1nM (publication pending). Assays have been completed by mixing cells producing AHL with "Receiver Cells" and plating on low concentrations of agar (~.3%). Confirmation of AHL production can be achieved by visualization of swarming (shown below).
User Reviews
UNIQ22bf06b13e26f729-partinfo-00000000-QINU UNIQ22bf06b13e26f729-partinfo-00000001-QINU