Difference between revisions of "Part:BBa J100382:Experience"

Latest revision as of 15:39, 12 April 2018

The final RFP/OD values for this riboswitch are shown as "#8" in the graph below. Ideally, the riboswitch would show high RFP/OD with xanthine and not the other conditions. However, the data suggest that the riboswitch actually remained theophylline specific.

To compare the theophylline induction of this riboswitch compared to the original RS-C, the fold-induction of each theophylline-specific riboswitch was calculated by dividing RFP/OD in the presence of theophylline by the RFP/OD without any ligand present. The chart below shows the fold-induction of wildtype (WT) RS-C and RS-C mutants #3, 7, 8 over a 10-hour experiment. RS-C mutant #8 has a lower induction than the WT RS-C or other RS-C mutants.

Applications of BBa_J100382

User Reviews

UNIQ1075bb539d628a23-partinfo-00000000-QINU UNIQ1075bb539d628a23-partinfo-00000001-QINU

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 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
+The final RFP/OD values for this riboswitch are shown as "#8" in the graph below. Ideally, the riboswitch would show high RFP/OD with xanthine and not the other conditions. However, the data suggest that the riboswitch actually remained theophylline specific.
-how you used this part and how it worked out.
+[[File:Final Fluorescence Values Graph.png|700px]]
+To compare the theophylline induction of this riboswitch compared to the original RS-C, the fold-induction of each theophylline-specific riboswitch was calculated by dividing RFP/OD in the presence of theophylline by the RFP/OD without any ligand present. The chart below shows the fold-induction of wildtype (WT) RS-C and RS-C mutants #3, 7, 8 over a 10-hour experiment. RS-C mutant #8 has a lower induction than the WT RS-C or other RS-C mutants.
+[[File:Improved Fold Induction Graph.png|700px]]
 ===Applications of BBa_J100382===