Difference between revisions of "Part:BBa J100381:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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The final RFP/OD values for this riboswitch are shown as "#7" in the graph below. Ideally, the riboswitch would show high RFP/OD with xanthine and not the other conditions. However, the data suggest that the riboswitch actually remained strongly theophylline specific.
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how you used this part and how it worked out.
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[[File:Final Fluorescence Values Graph.png|700px]]
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To compare the theophylline induction of this riboswitch compared to the original RS-C, the fold-induction of each theophylline-specific riboswitch was calculated by dividing RFP/OD in the presence of theophylline by the RFP/OD without any ligand present. The chart below shows the fold-induction of wildtype (WT) RS-C and RS-C mutants #3, 7, 8 over a 10-hour experiment. RS-C mutant #7 has a higher induction than the WT RS-C and mutant #8, but a lower induction than mutant #3.
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[[File:Improved Fold Induction Graph.png|700px]]
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===Applications of BBa_J100381===
 
===Applications of BBa_J100381===

Latest revision as of 15:38, 12 April 2018


The final RFP/OD values for this riboswitch are shown as "#7" in the graph below. Ideally, the riboswitch would show high RFP/OD with xanthine and not the other conditions. However, the data suggest that the riboswitch actually remained strongly theophylline specific.


Final Fluorescence Values Graph.png


To compare the theophylline induction of this riboswitch compared to the original RS-C, the fold-induction of each theophylline-specific riboswitch was calculated by dividing RFP/OD in the presence of theophylline by the RFP/OD without any ligand present. The chart below shows the fold-induction of wildtype (WT) RS-C and RS-C mutants #3, 7, 8 over a 10-hour experiment. RS-C mutant #7 has a higher induction than the WT RS-C and mutant #8, but a lower induction than mutant #3.


Improved Fold Induction Graph.png


Applications of BBa_J100381

User Reviews

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