Difference between revisions of "Part:BBa J100377:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The rClone Red plasmid was used instead of the tClone Red plasmid because RS-C, a translation riboswitch, is supposed to contain its own ribosome binding site (RBS) for it to function properly. rClone Red does not contain an RBS just upstream of the RFP sequence, so it allows for the proper testing of this RS-C mutant.
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The rClone Red plasmid was used instead of the tClone Red plasmid because RS-C, a translational riboswitch, is supposed to contain its own ribosome binding site (RBS) for it to function properly. rClone Red does not contain an RBS just upstream of the RFP sequence, so it allows for the proper testing of this RS-C mutant.
  
  

Latest revision as of 20:35, 9 April 2018


rClone Red with Riboswitch C Mutant #3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 662
    Illegal AgeI site found at 774
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rClone Red plasmid was used instead of the tClone Red plasmid because RS-C, a translational riboswitch, is supposed to contain its own ribosome binding site (RBS) for it to function properly. rClone Red does not contain an RBS just upstream of the RFP sequence, so it allows for the proper testing of this RS-C mutant.


Source

This part is the rClone Red plasmid (J119384) with an inserted mutated RS-C sequence.

References