Difference between revisions of "Part:BBa J100272:Experience"
Macampbell (Talk | contribs) |
Macampbell (Talk | contribs) |
||
| Line 4: | Line 4: | ||
<center> | <center> | ||
| − | [[File:rCloneRedv1_antiRBS.png| | + | [[File:rCloneRedv1_antiRBS.png|300px]] |
| − | </center> | + | </center> <br> |
To fix this problem, we mutated three codons so that they encoded the same 3 amino acids, but the resulting mRNA would not form the hairpin loop because we had disrupted the anti-RBS. <br> | To fix this problem, we mutated three codons so that they encoded the same 3 amino acids, but the resulting mRNA would not form the hairpin loop because we had disrupted the anti-RBS. <br> | ||
<center> | <center> | ||
| − | [[File:rCloneRedv2_antiRBS.png| | + | [[File:rCloneRedv2_antiRBS.png|300px]] |
| − | </center> | + | </center> <br> |
By removing the anti-RBS, the same RBS sequence now worked 10 times better than it had in rClone Red v1. | By removing the anti-RBS, the same RBS sequence now worked 10 times better than it had in rClone Red v1. | ||
<center> | <center> | ||
| − | [[File:rCloneRedv2_data.png| | + | [[File:rCloneRedv2_data.png|300px]] |
| − | </center> | + | </center> <br> |
We recommend everyone use J100272, rClone Red v2 as the preferred plasmid. | We recommend everyone use J100272, rClone Red v2 as the preferred plasmid. | ||
Revision as of 17:50, 10 March 2018
The problem with rClone Red v1 [J119384] was that the RBS we used was complementary to an "anti-RBS" located in RFP.
To fix this problem, we mutated three codons so that they encoded the same 3 amino acids, but the resulting mRNA would not form the hairpin loop because we had disrupted the anti-RBS.
By removing the anti-RBS, the same RBS sequence now worked 10 times better than it had in rClone Red v1.
We recommend everyone use J100272, rClone Red v2 as the preferred plasmid.
Applications of BBa_J100272
User Reviews
UNIQ7718c62b52c21c83-partinfo-00000000-QINU UNIQ7718c62b52c21c83-partinfo-00000001-QINU