Difference between revisions of "Part:BBa J100272:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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The problem with rClone Red v1 [[https://parts.igem.org/wiki/index.php/Part:BBa_J119384 J119384]] was that the RBS we used was complementary to an "anti-RBS" located in RFP. <br>
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how you used this part and how it worked out.
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<center>
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[[File:rCloneRedv1_antiRBS.png|500px]]
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To fix this problem, we mutated three codons so that they encoded the same 3 amino acids, but the resulting mRNA would not form the hairpin loop because we had disrupted the anti-RBS. <br>
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<center>
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[[File:rCloneRedv2_antiRBS.png|500px]]
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</center> 
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By removing the anti-RBS, the same RBS sequence now worked 10 times better than it had in rClone Red v1.  
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<center>
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[[File:rCloneRedv2_data.png|500px]]
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</center> 
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We recommend everyone use J100272, rClone Red v2 as the preferred plasmid.
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===Applications of BBa_J100272===
 
===Applications of BBa_J100272===

Revision as of 17:46, 10 March 2018


The problem with rClone Red v1 [J119384] was that the RBS we used was complementary to an "anti-RBS" located in RFP.

RCloneRedv1 antiRBS.png

To fix this problem, we mutated three codons so that they encoded the same 3 amino acids, but the resulting mRNA would not form the hairpin loop because we had disrupted the anti-RBS.

RCloneRedv2 antiRBS.png

By removing the anti-RBS, the same RBS sequence now worked 10 times better than it had in rClone Red v1.

RCloneRedv2 data.png

We recommend everyone use J100272, rClone Red v2 as the preferred plasmid.


Applications of BBa_J100272

User Reviews

UNIQf63588aca2cdca1c-partinfo-00000000-QINU UNIQf63588aca2cdca1c-partinfo-00000001-QINU