Difference between revisions of "Part:BBa J100356"

 
 
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This is an E. Coli bacterial promoter which is constitutive. Name of the promoter: j23101
 
This is an E. Coli bacterial promoter which is constitutive. Name of the promoter: j23101
 
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We chose this promoter because it has fewer than 60 base pairs and because the article said that it was effective when used in E.coli. We expect the promoter to efficiently generate red fluorescent colonies.
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 15:05, 22 February 2018


E. Coli bacterial promoter. The promoter is constitutive. Bacterial promoter name: j23101

This is an E. Coli bacterial promoter which is constitutive. Name of the promoter: j23101 We chose this promoter because it has fewer than 60 base pairs and because the article said that it was effective when used in E.coli. We expect the promoter to efficiently generate red fluorescent colonies. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 49
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 13
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 49
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 49
  • 1000
    COMPATIBLE WITH RFC[1000]