Difference between revisions of "Part:BBa I721003"
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'''Description'''<br>
 
'''Description'''<br>
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This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part.
+
This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I1721003.
  
 
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "
 
This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "
  
 
In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.
 
In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.
 +
 +
[[Image:BBa_1721003_1.jpg|thumb|left|Functional layout of this sequence]]
 +
[[Image:BBa_1721003_2.jpg|thumb|left|Overall Brown University iGEM 07 Design]]

Revision as of 22:55, 26 October 2007

Lead Binding Protein + Promoter (version b)

lead binding protein + promoter (part 8)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 479



Part BBa_1721003 is the Lead Binding Protein + Lead Promoter system

Description
This part is designed to enable Lead Detection in a cell. When lead enters the cell, it will couple with the Lead Binding protein, forming a dimer. This dimer will then bind to the Lead Promoter, which is also contained within this part, BBa_I1721003.

This part is not known to function. "PbrR691/promoter combinations in presence of lead nitrate give no GFP production and no AHL production compared to control. "

In the diagram below, this part contains three sections: A sequence for a Lead Binding Protein, a non-coding region, and a Lead Promoter.

Functional layout of this sequence
Overall Brown University iGEM 07 Design