Difference between revisions of "Part:BBa K2510029"

 
 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2510029 short</partinfo>
 
<partinfo>BBa_K2510029 short</partinfo>
 +
 +
 +
 +
 +
 +
<h3>Cell-free testing</h3>
 +
<P> This synthetic operator was part of the library that we used to test the activity of repressors caged with Dronpa </p>
 +
<img src="https://static.igem.org/mediawiki/2017/b/b1/96_conditions.png">
 +
 +
                            <b>Figure 1 : </b> the experiment conducted with the repressors caged with Dronpa
 +
                                       
 +
                                    </span>
 +
 +
                                      <img src="https://static.igem.org/mediawiki/2017/7/7d/Repression_strength_of_closed_state.png ">
 +
 +
                                      <b>Figure 2 : </b> An over view of the results of the cell-free experiment. Each
 +
                                        promoter was tested with its cognate repressors. Our results show that in 90% of our
 +
                                        constructs caging the repressors with Dronpa has increased the repression strength.
 +
                                        We have also obtained a wide linear range of repression strength-indicated by the red
 +
                                        slope-.
 +
                                    </span>
 +
                                    <img src="https://static.igem.org/mediawiki/2017/2/22/TetR_dronpa.png">
 +
                                    <img src="https://static.igem.org/mediawiki/2017/e/ed/P22c2_dronpa.png">
 +
                                    <img src="https://static.igem.org/mediawiki/2017/5/5e/HKCI_caged_dronpa.png">
 +
                                    <span  class="image-span text-center">
 +
                                            <b>Figure 3:</b> detailed Results of the cell-free experiment. Each promoter was
 +
                                            tested with its cognate repressors. <b>Top</b>: Testing with TetR caged with
 +
                                            either wt-Dronpa (BBa_K2510108) or a mutated version(BBa_K2510109)<b>Middle</b>:
 +
                                            Testing with P22c2 caged with either wt-Dronpa (BBa_K2510112) or a mutated
 +
                                            version(BBa_K2510113).<b>Bottom</b>: Testing with HKcI caged with either wt-
 +
                                            Dronpa (BBa_K2510110) or a mutated version(BBa_K2510111)          </span>
 +
 +
                                  <img src="https://static.igem.org/mediawiki/2017/5/5f/Wtvs_mut_repressors.png" >
 +
                                    <b>Figure 4:</b> the wtDronpa has a better control of HKC1 and C2P22 repressors while the mutDronpa has a better control of TetR repressors </span>
 +
  
 
qdkfjgs@
 
qdkfjgs@

Latest revision as of 03:56, 16 December 2017


O-TetR - T7 - O-TetR - O-P22C2 promoter



Cell-free testing

This synthetic operator was part of the library that we used to test the activity of repressors caged with Dronpa

<img src="96_conditions.png"> 

                            Figure 1 :  the experiment conducted with the repressors caged with Dronpa
                                        
                                   </span>

                                     <img src="Repression_strength_of_closed_state.png ">

                                      Figure 2 :  An over view of the results of the cell-free experiment. Each 
                                       promoter was tested with its cognate repressors. Our results show that in 90% of our 
                                       constructs caging the repressors with Dronpa has increased the repression strength. 
                                       We have also obtained a wide linear range of repression strength-indicated by the red 
                                       slope-.
                                   </span>
                                   <img src="TetR_dronpa.png">
                                   <img src="P22c2_dronpa.png">
                                    <img src="HKCI_caged_dronpa.png"> 
                                   
                                           Figure 3: detailed Results of the cell-free experiment. Each promoter was 
                                           tested with its cognate repressors. Top: Testing with TetR caged with 
                                           either wt-Dronpa (BBa_K2510108) or a mutated version(BBa_K2510109)Middle: 
                                           Testing with P22c2 caged with either wt-Dronpa (BBa_K2510112) or a mutated 
                                            version(BBa_K2510113).Bottom: Testing with HKcI caged with either wt-
                                           Dronpa (BBa_K2510110) or a mutated version(BBa_K2510111)           

                                  <img src="Wtvs_mut_repressors.png" >
                                   Figure 4: the wtDronpa has a better control of HKC1 and C2P22 repressors while the mutDronpa has a better control of TetR repressors </span>


qdkfjgs@

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]