Difference between revisions of "Part:BBa M50096:Design"
 
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<partinfo>BBa_M50096 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_M50096 SequenceAndFeatures</partinfo>
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EL-222 is a light-sensitive transcription factor that binds to DNA and drives transcription under blue light illumination. EL-222 consists of a LOV-HTH (helix-turn-helix) domain and VP16 activating unit. The LOV-HTH allows for light-gating of DNA binding; the LOV portion of the protein covers the HTH portion of the protein that binds DNA - under blue light exposure, the LOV domain changes conformation and reveals the HTH DNA binding site. The HTH binds to a sequence of c120 repeats. Once bound, the VP16 activating unit promotes transcription factors in the cell to drive genes downstream of the c120 repeats.
 
  
 
===Design Notes===
 
===Design Notes===
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Bacterial transcription factor
 
Bacterial transcription factor
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Supplemental Figures 2. "An optogenetic gene expression system with rapid activation and deactivation kinetics" Gardner et. al 2014, Nature Chemical Biology
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Supplemental Figures 2. "An optogenetic gene expression system with rapid activation and deactivation kinetics" Motta-Mena et. al 2014, Nature Chemical Biology
  
 
===References===
 
===References===
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"An optogenetic gene expression system with rapid activation and deactivation kinetics" Gardner et. al 2014, Nature Chemical Biology
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"An optogenetic gene expression system with rapid activation and deactivation kinetics" Motta-Mena et. al 2014, Nature Chemical Biology

Latest revision as of 01:24, 14 December 2017


EL-222 - Light-sensitive transcription factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 463
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 33
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

No changes were made to this sequence

Source

Bacterial transcription factor Supplemental Figures 2. "An optogenetic gene expression system with rapid activation and deactivation kinetics" Motta-Mena et. al 2014, Nature Chemical Biology

References

"An optogenetic gene expression system with rapid activation and deactivation kinetics" Motta-Mena et. al 2014, Nature Chemical Biology