Difference between revisions of "Part:BBa M50102"
 
Line 3: Line 3:
 
<partinfo>BBa_M50102 short</partinfo>
 
<partinfo>BBa_M50102 short</partinfo>
  
−
3 C120 repeats allows binding of EL-222 optogenetic transcription factor. EL-222 contains a VP-16 activator region that drives expression of genes downstream of C120 repeats. Here, we drove an Insulin p2A GFP downstream of c120 repeats.  
+
This light-inducible system uses the optogenetic transcription factor VPEL-222 to drive gene expression.3 C120 repeats allows binding of EL-222 domain of the transcription factor. Once bound, VPEL-222's VP-16 activator domain drives expression of genes downstream of C120 repeats. Here, we drove a preproinsulin gene.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:21, 14 December 2017


Light-inducible Insulin GFP

This light-inducible system uses the optogenetic transcription factor VPEL-222 to drive gene expression.3 C120 repeats allows binding of EL-222 domain of the transcription factor. Once bound, VPEL-222's VP-16 activator domain drives expression of genes downstream of C120 repeats. Here, we drove a preproinsulin gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]