Difference between revisions of "Part:BBa M50101"
 
Line 3: Line 3:
 
<partinfo>BBa_M50101 short</partinfo>
 
<partinfo>BBa_M50101 short</partinfo>
  
−
Sequence codes for a light-dependent transcription factor containing a nuclear localization sequence, VP-16 transcriptional activator, and EL-222 light-sensitive DNA-binding protein.
+
VPEL222 is a light-dependent transcription factor containing a nuclear localization sequence, VP-16 transcriptional activator, and EL-222 light-sensitive DNA-binding protein. This transcription factor can localize to the nucleus and binds to specific DNA sequences (c120 repeat sites) under blue light illumination. The VP-16 region promotes transcription of genes downstream from the c120 repeats once the transcription factor is bound.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:26, 13 December 2017


VPEL222 - Optogenetic Transcription Factor

VPEL222 is a light-dependent transcription factor containing a nuclear localization sequence, VP-16 transcriptional activator, and EL-222 light-sensitive DNA-binding protein. This transcription factor can localize to the nucleus and binds to specific DNA sequences (c120 repeat sites) under blue light illumination. The VP-16 region promotes transcription of genes downstream from the c120 repeats once the transcription factor is bound.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 945
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 925
    Illegal XhoI site found at 734
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 945
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 945
    Illegal NgoMIV site found at 304
  • 1000
    COMPATIBLE WITH RFC[1000]