Difference between revisions of "Part:BBa M50099:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | |||
| + | We modified this sequence to include a 6xHis Tag in the middle of the sequence, at residue 28 of the beta chain, immediately prior to an enzymatic cleavage site. His tagging allowed for subsequent measurement of preproinsulin/processed insulin using Western, ELISA, etc. | ||
===Source=== | ===Source=== | ||
| − | + | Human preproinsulin | |
===References=== | ===References=== | ||
| + | |||
| + | Sures, I., Goeddel, D.V., Gray, A., Ullrich, A. 1980. Nucleotide sequence of human preproinsulin complementary DNA. Science 208(4439) 57-9. | ||
Latest revision as of 11:04, 13 December 2017
Preproinsulin (w/ 6xHisTag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We modified this sequence to include a 6xHis Tag in the middle of the sequence, at residue 28 of the beta chain, immediately prior to an enzymatic cleavage site. His tagging allowed for subsequent measurement of preproinsulin/processed insulin using Western, ELISA, etc.
Source
Human preproinsulin
References
Sures, I., Goeddel, D.V., Gray, A., Ullrich, A. 1980. Nucleotide sequence of human preproinsulin complementary DNA. Science 208(4439) 57-9.