Difference between revisions of "Part:BBa K2270010"
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===Characterization===
 
===Characterization===
  
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[[Image:https://static.igem.org/mediawiki/parts/thumb/f/fe/AQA_Unesp--l.latis-pusp45-gfp.png/400px-AQA_Unesp--l.latis-pusp45-gfp.png|thumb|right|400px| <p align="justify"> '''Figure 2. '''''Growth curve and fluorescence from Lactococcus lactis cremoris cloned with Pusp45_GFP genes.</p>]]
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[[Image:400px-AQA_Unesp--l.latis-pusp45-gfp.png|thumb|right|400px| <p align="justify"> '''Figure 2. '''''Growth curve and fluorescence from Lactococcus lactis cremoris cloned with Pusp45_GFP genes.</p>]]
  
 
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Revision as of 01:31, 8 December 2017


superfolding GFP for Lactococcus lactis

Superfolding GFP codon-optimized for Bacillus subtilis. However, exhibits strong fluorescense signal in Lactococcus lactis. Used as protein report in L. lactis.

Usage and Biology

We (AQA_Unesp 2017) characterized the gene expression of sfGFP(Bs) during Lactococcus lactis growth under control of the usp45 promoter.


For more Details visit our [http://2017.igem.org/Team:AQA_Unesp/Results webpage]

Figure 1. Pellet of Lactococcus lactis cremoris cloned with Pusp45_GFP genes exited by a blue led transilluminator with filter.

Characterization

File:400px-AQA Unesp--l.latis-pusp45-gfp.png

Figure 2. Growth curve and fluorescence from Lactococcus lactis cremoris cloned with Pusp45_GFP genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13