Difference between revisions of "Part:BBa I737020"
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<partinfo>BBa_I737020 short</partinfo>
 
<partinfo>BBa_I737020 short</partinfo>
  
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This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 using XbaI and SpeI after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled.
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This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 using XbaI and SpeI after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled. It does however have the proper BioBrick prefix and suffix and can be assembled using XbaI and SpeI. Unfortunately this is not directional.
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This part has been sequenced and confirmed to be the beta-agarase from Pseudoalteromonas atlantica T6C. The sequence can be found here: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=109896332&db=Nucleotide&from=3202543&to=3204984&view=gbwithparts&RID=J2F5YTKG01R
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:15, 26 October 2007

PoPS->Agarase (Digests agar)

This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. We then cloned it into pSB1A2 using XbaI and SpeI after amplifying with primers that add the BioBrick prefix and suffix. Currently the gene has a EcorRI and a PstI site which limits how it can be assembled. It does however have the proper BioBrick prefix and suffix and can be assembled using XbaI and SpeI. Unfortunately this is not directional.


This part has been sequenced and confirmed to be the beta-agarase from Pseudoalteromonas atlantica T6C. The sequence can be found here: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=109896332&db=Nucleotide&from=3202543&to=3204984&view=gbwithparts&RID=J2F5YTKG01R

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1861
    Illegal NheI site found at 106
    Illegal NheI site found at 901
    Illegal PstI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1861
    Illegal XhoI site found at 1964
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1861
    Illegal PstI site found at 284
    Illegal AgeI site found at 368
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1319
    Illegal SapI.rc site found at 1717
    Illegal SapI.rc site found at 1795