Difference between revisions of "Part:BBa K2267028"

 
 
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<partinfo>BBa_K2267028 short</partinfo>
 
<partinfo>BBa_K2267028 short</partinfo>
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we Codon optimization Part:BBa_K319039 in G.xylinus
  
ok
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we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are
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plux1: https://parts.igem.org/Part:BBa_K2267029
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plux3: https://parts.igem.org/Part:BBa_K2267030
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plux4: https://parts.igem.org/Part:BBa_K2267031
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plux6: https://parts.igem.org/Part:BBa_K2267032
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plux8: https://parts.igem.org/Part:BBa_K2267046
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listed below.
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We linked gfp reporter genes
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luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043
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luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041
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luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042
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luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044
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luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045
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on them respectively to test the function of modified promoters.
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https://static.igem.org/mediawiki/2017/e/e6/T--TUST_China--part_part_results.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 04:00, 2 November 2017


GFP we Codon optimization Part:BBa_K319039 in G.xylinus

we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are

plux1: https://parts.igem.org/Part:BBa_K2267029

plux3: https://parts.igem.org/Part:BBa_K2267030

plux4: https://parts.igem.org/Part:BBa_K2267031

plux6: https://parts.igem.org/Part:BBa_K2267032

plux8: https://parts.igem.org/Part:BBa_K2267046

listed below. We linked gfp reporter genes luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043

luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041

luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042

luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044

luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045

on them respectively to test the function of modified promoters.

T--TUST_China--part_part_results.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 664