Difference between revisions of "Part:BBa K2510103"
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− | <p> This part is lacz-mutDronpa fusion, the output is β-galactosidase that can have higher activity levels by shining cyan [fig 2] shows that this copy shows a better control | + | <p> This part is lacz-mutDronpa fusion, the output is β-galactosidase that can have higher activity levels by shining cyan [fig 2] shows that this copy shows a better control than the original dronpa , the work flow of the β-galactosidase activity experiment is indicated in figure 1 </p> |
Revision as of 03:59, 2 November 2017
lacz-pdDronpa
This part is lacz-mutDronpa fusion, the output is β-galactosidase that can have higher activity levels by shining cyan [fig 2] shows that this copy shows a better control than the original dronpa , the work flow of the β-galactosidase activity experiment is indicated in figure 1
img id="fig2" src="" />
Figure 1: An overview of the experiment done to evaluate the activity of β-galactosidase-Dronpa fusion.
<img id="fig3" src="" />
Figure 2: X-Gal grayscale picture, testing the activity of β-galactosidase fusion with both wtDronpa and mutDronpa, indicating that β-galactosidase-mutDronpa fusion is more responsive to cyan light than the β-galactosidase-wtDronp.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]