Difference between revisions of "Part:BBa K2510103"

 
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<partinfo>BBa_K2510103 short</partinfo>
 
<partinfo>BBa_K2510103 short</partinfo>
  
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<p> This part is lacz-mutDronpa fusion, the output is β-galactosidase that can have higher activity levels by shining cyan [fig 2] shows that this copy shows a better control over than the original dronpa , the work flow of the  β-galactosidase activity experiment is indicated in figure 1  </p>
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img id="fig2" src="https://static.igem.org/mediawiki/2017/6/61/Aya_figure_13.png" />
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                                    <span  class="image-span text-center">
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                                            <b>Figure 1: </b>  An overview of the experiment done to evaluate the activity of β-galactosidase-Dronpa fusion.
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                                    </span>
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                            <img id="fig3" src="https://static.igem.org/mediawiki/2017/0/01/Aya_figure_14.png" />
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                                    <span  class="image-span text-center">
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                                            <b>Figure 2: </b> X-Gal grayscale picture, testing the activity of β-galactosidase fusion with both wtDronpa and mutDronpa, indicating that β-galactosidase-mutDronpa fusion is more responsive to cyan light than the β-galactosidase-wtDronp.
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                                    </span>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 03:58, 2 November 2017


lacz-pdDronpa


This part is lacz-mutDronpa fusion, the output is β-galactosidase that can have higher activity levels by shining cyan [fig 2] shows that this copy shows a better control over than the original dronpa , the work flow of the β-galactosidase activity experiment is indicated in figure 1 


img id="fig2" src="Aya_figure_13.png" /> 
 
                                   
                                           Figure 1:   An overview of the experiment done to evaluate the activity of β-galactosidase-Dronpa fusion. 
                                           
                                   

                            <img id="fig3" src="Aya_figure_14.png" /> 

                                   
                                           Figure 2:  X-Gal grayscale picture, testing the activity of β-galactosidase fusion with both wtDronpa and mutDronpa, indicating that β-galactosidase-mutDronpa fusion is more responsive to cyan light than the β-galactosidase-wtDronp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]