Difference between revisions of "Part:BBa K2194004"

Latest revision as of 03:57, 2 November 2017

Sulfate Transport System w/ Negative Feedback for Membrane Stress

This transcriptional unit codes for the genetic parts necessary to bind and transport chromate, sulfate, and related species across the cell membrane. BioBrick BBa_K2194004 is a composite part composed of negative feedback membrane stress promoter PgntK (BBa_K2194002), a synthetic RBS, cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), sulfate binding protein sbp (BBa_K2194001), and a synthetic terminator.

Biology and Usage

Refer to the registry pages for PgntK (BBa_K2194002), cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), and sulfate binding protein sbp (BBa_K2194001) for detailed biology.

Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates a higher rate of chromium import and increased toxicity to the cell.

Figure 1: Effect of Chromate on E. coli Growth

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1480
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2296
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Biology and Usage===
-Refer to the registry pages for <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002   BBa_K2194002]), a synthetic RBS, cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003   BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034   BBa_B0034]), sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001   BBa_K2194001]) for detailed biology.
+Refer to the registry pages for <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002   BBa_K2194002]), cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003   BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034   BBa_B0034]), and sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001   BBa_K2194001]) for detailed biology.
-Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI): lower growth rate for cysPUWA transformants in the presence of Cr(VI) may indicate the higher rate of chromium transport inside the cell.
+Figure 1 shows the effect of cysPUWA overexpression on the cells' susceptibility to Cr(VI). The lower growth rate for cysPUWA transformants in the presence of Cr(VI) indicates a higher rate of chromium import and increased toxicity to the cell.